HOME > EDICIONES > Año 1998, Volumen 48 - Número 1
Trabajos de Investigación
Métodos de extracción de quitina a partir de cáscara de camarón
|Araceli Pinelli Saavedra, Alma Rosa Toledo Guillén, Ingrid Rebeca Esquerra Brauer,
Alma Rosa Luviano Silva, Inocencio Higuera Ciapara
Centro de Investigaciones en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
Shrimp shell waste as a source of chitin biopolymers
Shrimp shell waste obtained from several industrial freezing- purchasing plants of Guaymas, Sonora, Méx., was studied as a source of value-added chitin biopolymers. In part I, the effect of different isolation conditions on the chitin yield and chemical characteristic, was investigated. Protem and mineral matter were removed with alkali and acid treatment respectively.
A 2x2x3 factorial a way of a completely randomized design was used in order to evaluate the effect of the process variables, namely, NaOH concentration (0.4 and 2%) during the deproteinization and HCl concentration (3 and 5%) carried out at 40,50 and 60°C. The best processing conditions were desproteinizaton with 2% NaOH, and demineralization with 5% HCl at 50°C, in terms of final ash and chitin content and yield.
In part II, a selection of methods of isolation of chitin and chitosan was studied in order to establish the best conditions for scaling up a process to pilot plant level. The processing conditions were selected from reported methods as well as from those defined in part I. Purity of chitin samples was determined in terms of residual protein, ash and chitin each one to produce high quality chitin (0,00% protein, 0,01 % ash, 99,99% chitin) and standard grade chitin (0,00% protein, 0,09% ash, 99,13% chitin).
Both products were considered as of adequate quality and their ; manufacture process could be scaled up by futher optimization of the processing conditions.
Key words: Shrimp shell, biopolymers, deproteinization, demineralization.
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ALAN-VE ISSN 0004-0622 - Depósito Legal: pp 199602DF83
Sociedad Latinoamericana de Nutrición
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