SUMMARY
Effects of dietary polyunsaturated fatty acids on adenylate cyclase, 5'nucleotidasa and Na⁺K⁺-ATPase activities in rat brain-plasma membrane
The incidence of polyunsaturated fatty acids (PUFA) in human nutrition is now generally accepted. As essential membrane components, PUFA may act as enzyme activity modulators. In this study, four different diets, in which PUFA type was the only modifying factor, were evaluated on 5' nucleotidase, adenylate cyclase and Na⁺/ K⁺ ATPase activities in rat brain plasma membranes. Animals fed the total PUFA deficient diet exhibited significant lower body weight and lower brain weight than did the control group. The specific activities of 5'nucleotidase and Na⁺/K⁺ATPase in brain plasma membrane were slightly modified by dietary PUFA. The catalytic unit of adenylate cyclase in total PUFA deficient animal s presented augmented enzyme activity and animals receiving diets deficient in n-6 PUFA showed reduced activity in relation to the control animals. Our results showed that the epinephrine receptors, in the case of adenylate cyclase are not modified by dietary PUFA, but rather the catalytic unit seems to be altered by dietary PUFA. These results can be partially explained by the fluidity that PUFA confers to membranes facilitating the proximity of enzyme-substrate. The physiological consequences of dietary PUFA incidence on enzyme activity needss further study.

Key words: PUFA deficiency, brain plasma membrane, adenylate cyclase, 5' nucleotidase, Na⁺K⁺ ATPase.



Recibido: 14/10/1997
Aceptado: 19/06/1998

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