HOME > EDICIONES > Año 2000, Volumen 50 - Número 1
Trabajos de Investigación
Modificación enzimática de las propiedades funcionales, nutricias y sensoriales de la soya para alimentación especial
Ana María Calderón de la Barca, Abraham Wall Medrano, Martín Jara Marini, Aarón F. González Cnrdova y Adolfo Ruíz Salazar
Dirección de Nutrición, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México.
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SUMMARY
Enzyrnatic modification of the functional, nutritional and sensorial properties of soy protein for special nutrition
Production of new protein-based products for special nutrition such as hypoallergenic infant formulas, fortified beverages and nutraceutics, require ideal ingredients. Protein ingredients were developed by enzymatic hydrolysis and methionine synthesis of soy protein. Hydrolysis was done at 4% (w/v) using porcine pancreatic enzymes (4% w/w), 50 °C, 6 h and pH 8. After drying powder was resuspended (20% w/v) and incubated with 7.6% (w/w) methionine methyl-ester, 1% (w/w) chymotrypsin and 3 M glycerol. 37 °C. 3h and pH 7. Hydrolysates were fractionated by ultrafiltration (VF) before and after enrichment (E):FI>10,10>FII>3and 3>FIII>1 kDa. Functional propertics, amino acid content anti-physiological factor activities and antigenicity were assayed for all the UF fractions and the soybean meal. Protein quality bioassay and sensorial test of an non-enriched fraction and an enriched fraction were performed. Functional properties were positively modified by hydrolysis and synthesis by using a minimum time and methionine added for the last reaction. After UF all the fractions under 10 kDa showed 100% solubility (pH 4 and 7). good clarity, acceptable foam capacity and negligible antigenicity and antiphysiological activities. Additionally, methionine enrichment enhanced their nutritional value, upgrading sulfur amino acid requirements for infants and adults. Because functionality and nutritional value FIII-E could be used for hypoallergenic infant formulas, FII-E for fortified soluble formulas and nutraceutics and FI-E for a semi-solid baby food.
Key words: Soyprotein, enzymatic modification.
Recibido: 29/06/1999
Aceptado: 10/12/1999
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PRIVACIDAD | ACCESIBILIDAD
ALAN-VE ISSN 0004-0622 - Depósito Legal: pp 199602DF83
Sociedad Latinoamericana de Nutrición
Producción editorial en Venezuela: Capítulo Venezolano - RIF: J-30843129-0
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