HOME > EDICIONES > Año 2009, Volumen 59 - Número 1
Trabajos de Investigación
Contenido de sulforafano (1-isotiocianato-4-(metilsulfinil)-butano) en vegetales crucíferos
Olga N. Campas-Baypoli, Carolina Bueno-Solano, Diana M. Martínez-Ibarra, Francisco Camacho-Gil, Alma G. Villa-Lerma, Jesús R. Rodríguez-Núñez, Jaime López-Cervantes, Dalia I. Sánchez-Machado
Instituto Tecnológico de Sonora, Cd. Obregón, Sonora, México
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SUMMARY
Sulforaphane (1-isothiocyanato-4-(methylsulfinyl)-butane) content in cruciferous vegetables
Sulforaphane is an isothiocyanate which has antimicrobial and anticarcinogenic properties, this compound is found in a wide variety of plants from genus Brassica oleracea, being the most important broccoli and cabbage. The objective of this research was to quantify sulforaphane in the edible parts of broccoli and cabbage leaves by high-performance liquid chromatography (HPLC). Sample preparation for the quantification of sulforaphane include the conversion of glucoraphanin to sulforaphane (45 ± 2 °C for 2.5 h), extracted with dichloromethane, purification of the extract in columns of solid phase extraction and detection by HPLC- UV. Sulforaphane concentration in broccoli is in the range of 214 µg/g DW (stems) to 499 µg/g DW (inflorescences). The purple cabbage (101.99 µg/g DW) has values greater than the green cabbage (7.58 µg/g DW). The inflorescences of broccoli and red cabbage leaves are rich in sulforaphane.
Key words: Sulforaphane, glucoraphanin, broccoli, cabbage, HPLC.
Recibido: 04/11/2008
Aceptado: 03/12/2008
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PRIVACIDAD | ACCESIBILIDAD
ALAN-VE ISSN 0004-0622 - Depósito Legal: pp 199602DF83
Sociedad Latinoamericana de Nutrición
Producción editorial en Venezuela: Capítulo Venezolano - RIF: J-30843129-0
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