1 Universidad Autónoma del Estado de México, Toluca, México.
Introduction: Sweeteners are currently more than 4000 commercial products, their use is approved by international organizations, but its use at an early age is still controversial. The aim of the study was to evaluate the use of sweeteners in newly weaned mice and its influence on lymphocytes from peripheral lymphoid organs. Methodology: Were used 32 CD1 male mice of 21 days old divided into 4 groups: i) Control (CL), ii) Sucrose (SUC), iii) Sucralose (SUCLOS) and Stevia (ST). 10g of sucrose and 1g of sucralose and stevia were administered diluted in 240mL of water; water and food consumed ad libitud (3th-9th weeks of life). Body weight, body mass index and blood glucose was recorded. The percentage of blood lymphocytes, spleen and Peyer’s patch was obtained and the carbonylated protein it was measured. Results: The weight (F=1.33, p<0.282), BMI (F =1.13, p<0.353) and blood glucose (F=2.59, p<0.072) at the end of the 6 weeks of treatment remained unchanged. The differences in the percentages of lymphocytes found: decreased in blood (CLSUC p<0.001 and ST-SAC p<0.014) and Peyer’s Patches (F=8.76, p<0.001); increased in the spleen (CL-SUC p<0.035) at expense of SUC groups (p<0.002) and ST (p<0.001). Carbonylated proteins were significantly modified in spleen (F=8.65, p<0.001) and Blood (F=29.20, p<0.001) lymphocytes. Conclusions: Protein damage was apparent with sucrose consumption and sucralose, decreasing consumption with stevia; suggesting that Stevia consumption does not increase early carbonylated protein concentration, situation which occurs with consumption of sucrose and sucralose.